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CD40L stimulation enhances the ability of conventional metaphase cytogenetics to detect chromosome aberrations in B‐cell chronic lymphocytic leukaemia cells
Author(s) -
Buhmann Raymund,
Kurzeder Christian,
Rehklau Jutta,
Westhaus Doreen,
Bursch Sabina,
Hiddemann Wolfgang,
Haferlach Torsten,
Hallek Michael,
Schoch Claudia
Publication year - 2002
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2002.03719.x
Subject(s) - cytogenetics , metaphase , biology , fluorescence in situ hybridization , microbiology and biotechnology , chromosome , cancer research , genetics , gene
Summary. Conventional metaphase cytogenetics underestimates the frequency of specific chromosome aberrations in B‐cell chronic lymphocytic leukaemia (B‐CLL) as a result of the very low proliferative activity of these cells in vitro . New molecular approaches, such as fluorescence in situ hybridization (FISH) or comparative genomic hybridization (CGH), may circumvent this problem, at least in part, but these techniques are either strongly dependent on the knowledge of candidate regions or detect only unbalanced aberrations. In the present study, we analysed 27 B‐CLL peripheral blood samples by metaphase cytogenetics after CD40 ligand (CD40L)‐induced cell cycle stimulation. In comparison with the simultaneous use of B‐cell mitogens such as 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA), lipopolysaccharide (LPS) and pokeweed mitogen (PWM), CD40L stimulation of B‐CLL cells induced a threefold increase in metaphases amenable to analysis by conventional cytogenetics. The analysis of these metaphases confirmed all genetic abnormalities detected by FISH. Moreover, CD40L‐enhanced cytogenetics revealed complex karyotypic aberrations in 11 out of 27 patients (41%). In one case, a balanced translocation t(11;16)(p15;p13.1), so far unreported in B‐CLL, was detected. Taken together, the results of our study show the potential of CD40L‐enhanced metaphase cytogenetics to detect more and new chromosome aberrations in B‐CLL.