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High P‐glycoprotein‐mediated export observed in patients with a history of idiopathic thrombocytopenic purpura
Author(s) -
Levy Adam S.,
CunninghamRundles Susanna,
Mazza BethAnne,
Simm Maciej,
Gorlick Richard,
Bussel James
Publication year - 2002
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2002.03709.x
Subject(s) - evans syndrome , thrombocytopenic purpura , peripheral blood mononuclear cell , immunology , medicine , flow cytometry , glycoprotein , autoimmune disease , autoimmunity , platelet , biology , immune system , microbiology and biotechnology , antibody , biochemistry , autoimmune hemolytic anemia , in vitro
Summary. Studies have suggested that high P‐glycoprotein expression in lymphocytes from patients with autoimmune disorders may affect disease outcome. Idiopathic thrombocytopenic purpura (ITP) and Evans' syndrome are widely thought to be autoimmune processes, however, the precise mechanisms remain unknown. Peripheral blood mononuclear cells from patients with refractory or recurrent ITP or Evans' syndrome were studied using the rhodamine 123 flow cytometric assay to investigate functional export levels. Lymphocytes from ITP and Evans' syndrome patients showed a significantly decreased ability to retain rhodamine, suggesting increased export protein function. Reverse transcription polymerase chain reaction distinguished P‐glycoprotein as the likely export protein.