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The role of common gamma chain in human monocytes in vivo ; evaluation from the studies of X‐linked severe combined immunodeficiency (X‐SCID) carriers and X‐SCID patients who underwent cord blood stem cell transplantation
Author(s) -
Ariga Tadashi,
Yamaguchi Koji,
Yoshida Jukei,
Miyanoshita Akihiko,
Watanabe Toshihide,
Date Tadashi,
Miura June–ichi,
Kumaki Satoru,
Ishii Naoto,
Sakiyama Yukio
Publication year - 2002
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2002.03679.x
Subject(s) - severe combined immunodeficiency , immunology , immunodeficiency , in vivo , biology , transplantation , monocyte , flow cytometry , microbiology and biotechnology , cancer research , medicine , immune system , genetics
Summary. Expression of common gamma chain (γc) on monocytes was studied in five carriers of X‐linked severe combined immunodeficiency (X‐SCID) and two X‐SCID patients who underwent cord blood stem cell transplantation (CBSCT). Flow cytometric analysis revealed that both γc‐negative and positive monocytes co‐existed in X‐SCID carriers, whereas no γc‐negative T, B or NK cells were observed in them. Clonal analysis and reverse transcription polymerase chain reaction studies revealed that 13·2–45·0% of monocytes from these carriers expressed the mutant γc message. X‐SCID patients who received CBSCT persistently possessed the majority of γc‐negative monocytes with a good clinical course. These results, together, may indicate that γc is not essential for monocyte development/function in vivo .