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DNA microarray analysis of T cell‐type lymphoproliferative disease of granular lymphocytes
Author(s) -
Makishima Hideki,
Ishida Fumihiro,
Ito Toshiro,
Kitano Kiyoshi,
Ueno Shuichi,
Ohmine Ken,
Yamashita Yoshihiro,
Ota Jun,
Ota Masao,
Yamauchi Kazuyoshi,
Mano Hiroyuki
Publication year - 2002
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2002.03646.x
Subject(s) - biology , cd8 , t cell , immunology , microarray , lymphoproliferative disorders , monoclonal antibody , microarray analysis techniques , gene , microbiology and biotechnology , gene expression , immune system , antibody , genetics , lymphoma
Summary. Lymphoproliferative disease of granular lympho‐ cytes (LDGL) is characterized by the clonal proliferationoflarge granular lymphocytes of either T‐ or natural killer cell origin. To better understand the nature of T cell‐type LDGL, we purified the CD4 – CD8 + proliferative fractions from LDGL patients ( n =4) and the surface marker‐matched T cells isolated from healthy volunteers ( n =4), and compared the expression profiles of 3456 genes using DNA microarray. Through this analysis, we identified a total of six genes whose expression was active in the LDGL T cells, but silent in the normal ones. Interestingly, expression of the gene for interleukin (IL) 1β was specific to LDGL T cells, which was further confirmed by the examination of the serum level of IL‐1β protein. Given its important role in inflammatory reactions, the disease‐specific expression of IL‐1β may have a causative relationship with the LDGL‐ associated rheumatoidarthritis. Spectratyping analysis of the T‐cell receptor repertoire also proved the monoclonal or oligoclonal natureof LDGL cells. These data have shown that microarray analysis with a purified T‐cell subset is an efficient approach to investigate the pathological condition of Tcell‐type LDGL.