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Characterization of granulocyte colony‐stimulating factor receptor expressed on human lymphocytes
Author(s) -
Morikawa Keiko,
Morikawa Shigeru,
Nakamura Masahiko,
Miyawaki Toshio
Publication year - 2002
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2002.03574.x
Subject(s) - biology , microbiology and biotechnology , b cell , immunoglobulin d , granulocyte colony stimulating factor receptor , population , bone marrow , immunofluorescence , immunology , antibody , haematopoiesis , medicine , stem cell , environmental health
Summary. We have studied the characterization of granulocyte colony‐stimulating factor receptor (G‐CSFR) in human lymphocytes. About one‐third to one‐quarter of the B lymphocytes from peripheral B‐cell sources displayed G‐CSF binding on the two‐colour immunofluorescence study. The rate of G‐CSFR‐expressing (G‐CSFR + ) B cells was higher in bone marrow and cord blood than in peripheral blood, spleen and tonsil. G‐CSFR expression was greater in the surface immunoglobulin D (IgD)‐positive (sIgD + ) B‐cell population, but scarce in the sIgD – B‐cell population. In tonsil, G‐CSFR + B cells were present among the cells with naive B and germinal‐centre B phenotypes, but those with memory B phenotype were rarely found on triple‐colour immunofluorescence analysis. Mitogen‐activated, but not resting, T lymphocytes also showed G‐CSF binding. Several continuous T‐ and B‐cell lines expressed functional G‐CSFR, because the addition of G‐CSF enhanced the proliferative response of these cell lines. A sequence analysis of G‐CSFR mRNA isoforms obtained from the T and B cells revealed that G‐CSFR was derived from class I and class IV mRNA. Our results indicated that G‐CSFR was constitutively expressed on the B‐cell surface and was inducible in T cells.