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Blast crisis of Philadelphia chromosome‐positive chronic myeloid leukaemia carrying micro‐bcr breakpoint (e19a2 and e191a)
Author(s) -
Ohsaka Akimichi,
Hoshino Shigeru,
Kobayashi Masaru,
Kudo Hideyuki,
Kawaguchi Ryuji
Publication year - 2002
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2002.03570.x
Subject(s) - breakpoint cluster region , breakpoint , philadelphia chromosome , cancer research , chromosomal translocation , bone marrow , biology , medicine , immunology , genetics , gene
Summary. We describe two Philadelphia chromosome‐positive chronic myeloid leukaemia (Ph‐positive CML) patients carrying micro‐bcr (µ‐bcr) breakpoint, who developed blast crisis within 5 years of diagnosis. Reverse transcription polymerase chain reaction analysis of bone marrow cells using primers specific for the p210 BCR–ABL fusion transcript showed aberrant large‐sized bands in both cases (986 bp in patient 1 and 1031 bp in patient 2). Sequencing analysis of these products revealed BCR–ABL fusion transcripts with e19a2 in patient 1 and e191a in patient 2. These findings suggest that CML carrying µ‐bcr breakpoint may exhibit a similar clinical course to classical CML, and that it may not be a mild form of the disease.