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Induction of CD94/NKG2A expression on T cells in mixed lymphocyte culture by CD14 + cells from granulocyte colony‐stimulating factor‐mobilized peripheral blood mononuclear cells
Author(s) -
Tanaka Junji,
Tutumi Yutaka,
Li Zhang,
Katho Naoko,
Sugita Junichi,
Mori Akio,
Toyoshima Nobuyasu,
Ota Shuichi,
Kobayashi Masanobu,
Kasai Masaharu,
Asaka Masahiro,
Imamura Masahiro
Publication year - 2002
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2002.03525.x
Subject(s) - cd14 , peripheral blood mononuclear cell , cd8 , immunology , lymphocyte , biology , microbiology and biotechnology , chemistry , antigen , immune system , in vitro , biochemistry
Summary. We report the increased expression of inhibitory natural killer (NK) cell receptor, CD94/NKG2A, for human leucocyte antigen (HLA) class I on CD8 + T cells in granulocyte colony‐stimulating factor‐mobilized peripheral blood mononuclear cells (G‐PBMC) after mixed lymphocyte culture (MLC). The addition of purified CD14 + cells to CD14‐depleted G‐PBMC‐induced CD94/NKG2A expression in a dose‐dependent fashion; however, this enhancing effect was inhibited by the membrane between responder cells and CD14 + cells. Therefore, CD14 + cells in G‐PBMC induce CD94/NKG2A expression on CD8 + T cells, which in turn appear to downregulate alloresponses after allogeneic peripheral blood stem cell transplantation.