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Qualitative and quantitative polymerase chain reaction detection of the residual myeloma cell contamination after positive selection of CD34 + cells with small‐ and large‐scale Miltenyi cell sorting system
Author(s) -
Voena Claudia,
Locatelli Giovanni,
Castellino Claudia,
Omedè Paola,
Ladetto Marco,
Zappone Elisabetta,
Milani Raffaella,
Perfetti Vittorio,
Boccadoro Mario,
Pileri Alessandro,
Lusso Paolo,
Villa Chiara,
Malnati Mauro,
Corradini Paolo
Publication year - 2002
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2002.03448.x
Subject(s) - multiple myeloma , polymerase chain reaction , cell sorting , sorting , minimal residual disease , cd34 , real time polymerase chain reaction , microbiology and biotechnology , biology , immunology , gene , flow cytometry , genetics , bone marrow , stem cell , computer science , programming language
Summary. The purging efficacy of the Miltenyi sorting system was evaluated by qualitative and TaqMan quantitative polymerase chain reaction (PCR) in myeloma patients, using immunoglobulin genes. After small‐scale selection, qualitative PCR showed that in 6 of 12 leukaphereses myeloma cells were no longer detectable. Envisaging a possible clinical application, the leukaphereses from three patients underwent large‐scale selection. Qualitative PCR showed that myeloma cells were still detectable. Quantitative PCR, performed in two patients, showed a tumour depletion of␣1 and 2 logs respectively. Although numbers are small, the␣promising results obtained with small‐scale selection were␣not reproduced in large‐scale experiments.