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Tumour necrosis factor‐α‐induced apoptosis in cord blood T lymphocytes: involvement of both tumour necrosis factor receptor types 1 and 2
Author(s) -
Yang YaChien,
Hsu TsueyYing,
Chen JenYang,
Yang CzauSiung,
Lin RongHwa
Publication year - 2001
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2001.03090.x
Subject(s) - cord blood , apoptosis , tumor necrosis factor alpha , necrosis , immunology , medicine , cd8 , cytokine , programmed cell death , biology , immune system , biochemistry
Cord blood T cells are much more likely to be induced to apoptosis in vitro than adult T cells. Nevertheless, the expression of Fas is markedly lower on cord blood lymphocytes than on peripheral blood lymphocytes. In the current investigation, we determined the capacity of tumour necrosis factor‐α (TNF‐α) to induce apoptosis in human naïve T cells in cord blood, and assessed the roles of two distinct TNF receptors (TNFRs) in mediating death signals. After activation, cord blood T cells were sensitive to TNF‐α‐induced apoptosis, and interleukin 2 (IL‐2) could prevent this apoptotic response. Both TNFR1 (p55) and TNFR2 (p75) expressed on activated cord blood T cells were able to transmit apoptotic signals. Moreover, a synergistic effect was observed by a combination of TNFR1‐ and TNFR2‐signals. Additionally, CD4 + T cells showed higher sensitivity to TNFR‐mediated apoptosis than CD8 + T cells. These data suggest that TNF‐α probably is a mediator of apoptosis in cord blood T cells in vivo and may contribute to the low incidence of graft‐versus‐host disease in cord blood transplantation.