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Deregulation of immunoglobulin gene transcription in the Hodgkin–Reed Sternberg cell line L1236
Author(s) -
Re Daniel,
StaratschekJox Andrea,
Holtick Udo,
Diehl Volker,
Wolf Jürgen
Publication year - 2001
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2001.03086.x
Subject(s) - gene , biology , microbiology and biotechnology , reed–sternberg cell , immunoglobulin gene , gene expression , transcription (linguistics) , regulation of gene expression , immunoglobulin heavy chain , mutation , genetics , immunology , lymphoma , linguistics , philosophy , hodgkin lymphoma
Hodgkin–Reed Sternberg (H‐RS) cells harbour clonal immunoglobulin gene (Ig) rearrangements in almost all cases of classical Hodgkin's disease but lack Ig gene expression. In the H‐RS cell line L1236, a somatic mutation of the Ig heavy‐chain gene promoter octamer motif has been described as a putative reason for absence of Ig gene expression. We addressed transcriptional activity of this mutated promoter by performing reporter gene studies and gel retardation assays. The results showed that the mutation outside the coding region of the rearranged Ig gene in L1236 cells leads to downregulation of Ig gene expression in H‐RS cells.