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Cell cycle analysis and expression of cell cycle regulator genes in myeloma cells overexpressing cyclin D1
Author(s) -
Yata Kenichiro,
Sadahira Yoshito,
Otsuki Takemi,
Sakaguchi Haruko,
Isozaki Yumika,
Uno Masako,
Kurebayashi Junichi,
Fujii Tomohiro,
Eda Sakura,
Ueki Ayako,
Yawata Yoshihito,
Yamada Osamu,
Sugihara Takashi
Publication year - 2001
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2001.02990.x
Subject(s) - cyclin d1 , cyclin d , cyclin b , biology , cell cycle , cyclin , cyclin dependent kinase , cyclin a2 , cancer research , cyclin a , cyclin d2 , microbiology and biotechnology , cell , genetics
Among the recently discovered myeloma‐specific gene alterations associated with chromosomal translocations, cyclin D1/PRAD1/Bcl‐1 overexpression caused by t(11;14)(q13;q32) is considered to be the most frequent in myeloma patients and cell lines, and may be a prognostic factor clinically. To elucidate the cellular biological role of overexpressed cyclin D1 in myeloma cells, we examined the mRNA expression levels of cell cycle regulators including three cyclin Ds, cyclin‐dependent kinase inhibitors (CDK‐Is) and accelerators. Cyclin D1 overexpression was clearly demonstrated in the lines with abnormal 11q13 and associated with overexpression of S and G 2 accelerator genes. The cyclin D1 ‐overexpressing lines tended to have a shortened G 1 phase compared with the non‐expressing lines. In addition, artificial silencing using antisense oligonucleotides for cyclin D1 suppressed the growth rate of some but not all cyclin D1 ‐overexpressing cells. These results indicate that overexpression of cyclin D1 caused by cytogenetic abnormalities may make cells progress through the cell cycle rapidly, but it seems that other factors such as cyclin D2 and translocation‐related genes affect the cell cycle progression in myeloma cells.