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Mutation screening of the c‐MYB negative regulatory domain in acute and chronic myeloid leukaemia
Author(s) -
Lutwyche Jodi K.,
Keough Rebecca A.,
Hughes Timothy P.,
Gonda Thomas J.
Publication year - 2001
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2001.02966.x
Subject(s) - myb , mutation , biology , myeloid , gene , cancer research , pathogenesis , genetics , transcription factor , immunology
Over‐expression of the c‐myb gene and expression of activated forms of myb are known to transform haemopoietic cells, particularly cells of the myeloid lineage. Truncations or mutations that disrupt the negative regulatory domain (NRD) of the Myb protein confer an increased ability to transform cells. Although it has proved difficult to link mutations in c‐MYB to human leukaemia, no studies investigating the presence of mutations within the c‐MYB NRD have been reported. Therefore, we have performed mutational analysis of this region, using polymerase chain reaction–single‐stranded conformation polymorphism and sequence analysis, in 26 patients with acute or chronic myeloid leukaemia. No mutations were detected, indicating that mutation of this region of the Myb protein is not common in the pathogenesis or progression of these diseases.