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Rapid genotyping of human platelet antigen 5 with fluorophore‐labelled hybridization probes on the LightCycler
Author(s) -
Kretzschmar Evelyne,
Von Ahsen Nicolas,
Trobisch Heiner
Publication year - 2001
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2001.02951.x
Subject(s) - genotyping , neonatal alloimmune thrombocytopenia , typing , polymerase chain reaction , antigen , thrombocytopenic purpura , immunology , platelet , biology , medicine , genotype , gene , genetics , fetus , pregnancy
Genotyping of human platelet antigens (HPAs) can be useful for the diagnosis and therapy of alloimmune thrombocytopenic syndromes such as neonatal alloimmune thrombocytopenic purpura, post‐transfusion purpura and refractoriness to platelet transfusion therapy. We developed a single‐tube method for HPA‐5 genotyping on the LightCycler that combines rapid‐cycle polymerase chain reaction with allele‐specific fluorescent probe melting for mutation detection. Our method is fast, robust and suitable for routine HPA‐5 typing. This work extends recent studies on HPA‐1 typing using the LightCycler.