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Identification of both myeloid CD11c + and lymphoid CD11c − dendritic cell subsets in cord blood
Author(s) -
Borràs Francesc E.,
Matthews Nick C.,
Lowdell Mark W.,
Navarrete Cristina V.
Publication year - 2001
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2001.02840.x
Subject(s) - cd11c , myeloid , immunology , dendritic cell , cord blood , cd33 , biology , lymphatic system , cd80 , t cell , interleukin 3 receptor , follicular dendritic cells , antigen presenting cell , phenotype , antigen , stem cell , immune system , cytotoxic t cell , cd40 , microbiology and biotechnology , cd34 , in vitro , gene , biochemistry
Dendritic cells (DCs) are the most potent antigen‐presenting cells described to date. In human peripheral blood, both myeloid and lymphoid subsets of DCs have been identified. In contrast, cord blood (CB) DCs have recently been described as being exclusively of the immature CD11c − lymphoid DC subset. Using an alternative method of enrichment, based on a negative selection system, both lymphoid (HLA‐DR + CD123 +++ CD11c − CD33 − ) and myeloid (HLA‐DR ++ CD123 + CD11c + CD33 + ) DCs were identified in CB. Although the majority of CB DCs showed a lymphoid phenotype, a significant number of CD11c + myeloid DCs (25·6% ± 14·5%, n = 13) were also present. Other markers, such as CD80 and CD83, were negative in both subsets. Analyses of the allostimulatory capacity of both subsets showed that freshly isolated CB lymphoid DCs failed to induce a potent allostimulation of naive CB T cells. These features are therefore consistent with previous work reporting an immature phenotype for lymphoid DCs in adult blood. The significance of the inverted CD11c + /CD11c − ratio observed in CB DCs (1:3) with respect to adult blood DCs (3:1) remains to be explained.