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A cautionary tale: how to delete mouse haemopoietic stem cells with busulphan
Author(s) -
Jopling Chris,
Rosendaal Martin
Publication year - 2001
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2001.02825.x
Subject(s) - stem cell , haematopoiesis , busulfan , biology , bone marrow , immunology , medicine , hematopoietic stem cell transplantation , microbiology and biotechnology
In this study treating mice with the ‘correct’ dose of busulphan did not necessarily destroy all haematopoietic stem cells. In certain circumstances host stem cells survived undetected and subsequently resumed haemopoiesis. This may apply to the use of busulphan clinically. We found that the following conditions determined the deletion of mouse stem cells using busulphan: (1) graft size – grafting more than 10 6 marrow cells (∼0·3% of the animal's stem cells) concealed the survival of stem cells; (2) dose of busulphan – insufficient busulphan did not kill all host stem cells; (3) old or improperly stored busulphan failed to delete all host stem cells; furthermore (4) the survival of host stem cells should be assessed by typing many kinds of circulating cells; and (5) tests should be carried out to determine if busulphan has killed all host stem cells by typing circulating blood cells at appropriate intervals.