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Residual risk of transfusion in Ghana
Author(s) -
Candotti Daniel,
Sarkodie Francis,
Allain JeanPierre
Publication year - 2001
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2001.02679.x
Subject(s) - residual risk , hepatitis c virus , volunteer , medicine , blood transfusion , hepatitis c , virology , virus , immunology , human immunodeficiency virus (hiv) , viral disease , hepacivirus , rna , polymerase chain reaction , flaviviridae , antigen , biology , gene , biochemistry , agronomy
Plasma samples from replacement and volunteer blood donors in Kumasi, Ghana were pooled and tested using a duplex human immunodeficiency virus (HIV) and hepatitis C virus (HCV) RNA detection method. Individual plasmas constitutive of reactive pools were confirmed using reverse transcription‐polymerase chain reaction. HIV and HCV infections were significantly higher in 1569 replacement donors than in 1169 volunteers; 2·4 and 1·7 versus 0·3 and 0·7% respectively ( P < 0·01). Two duplex RNA‐positive plasma pools contained a confirmed/seronegative HIV or HCV RNA individual plasma. The residual post‐transfusion risk of HIV and HCV infection of blood collected from replacement blood donors ranged between 1:260 and 1:16 393 after screening for anti‐HIV, p24 antigen and anti‐HCV. These data indicate that in high‐prevalence HIV and HCV blood donor populations, a substantial residual post‐transfusion risk of infection remains. This risk might be reduced by collecting blood in younger volunteer donors or by genomic screening.