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Flow cytometry and the study of central nervous disease in patients with acute leukaemia
Author(s) -
Subirá D.,
Castañón S.,
Román A.,
Aceituno E.,
JiménezGarófano C.,
Jiménez A.,
García R.,
Bernácer M.
Publication year - 2001
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2001.02505.x
Subject(s) - flow cytometry , cerebrospinal fluid , pathology , cytology , central nervous system , medicine , disease , immunology
Central nervous system (CNS) leukaemia is still a matter of debate and new technologies are required to improve the classic morphological definition. One hundred and sixty‐eight cerebrospinal fluid (CSF) samples from 31 patients with acute leukaemia were analysed by flow cytometry and conventional cytology. Concordant positive and negative findings were found in 158 samples but 10 produced discrepant results. Cytology seemed to offer more precise information in one CSF sample and flow cytometric accuracy could be demonstrated in five samples. We conclude that flow cytometry is of great help in confirming CNS leukaemia and eliminating other conditions. Therefore, leukaemic patients can benefit from double cytological and flow cytometric CSF studies.