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Involvement of natural killer cells in patients with myelodysplastic syndrome carrying monosomy 7 revealed by the application of fluorescence in situ hybridization to cells collected by means of fluorescence‐activated cell sorting
Author(s) -
Miura Ikuo,
Kobayashi Yoshimi,
Takahashi Naoto,
Saitoh Kohki,
Miura Akira B.
Publication year - 2000
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2000.02294.x
Subject(s) - monosomy , fluorescence in situ hybridization , cd34 , cd19 , cd3 , biology , microbiology and biotechnology , progenitor cell , chromosome 7 (human) , cancer research , pathology , stem cell , immunology , medicine , karyotype , cd8 , chromosome , antigen , flow cytometry , genetics , gene
Monosomy 7 is the most frequent chromosome abnormality among patients with secondary myelodysplastic syndrome (MDS). We used fluorescence in situ hybridization (FISH) and fluorescence‐activated cell sorting (FACS) in order to clarify the lineage involvement. Four patients, three with de novo MDS and one with secondary MDS, were enrolled in this study. Monosomy 7 was observed in pluripotent stem cells (CD34 + Thy‐1 + ), and in B (CD34 + CD19 + ) and T/natural killer (NK) progenitors (CD34 + CD7 + ). The number of abnormal cells of B (CD19 + ) and T (CD3 + ) cells was below the cut‐off value, but approximately 60% of the NK cells (CD3‐CD56 + ) contained monosomy 7 in three of the patients.