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Kell is not restricted to the erythropoietic lineage but is also expressed on myeloid progenitor cells
Author(s) -
Wagner T.,
Berer A.,
Lanzer G.,
Geissler K.
Publication year - 2000
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2000.02195.x
Subject(s) - myelopoiesis , erythropoiesis , myeloid , progenitor cell , peripheral blood mononuclear cell , antibody , immunology , biology , antigen , haematopoiesis , granulocyte , progenitor , cfu gm , microbiology and biotechnology , stem cell , medicine , in vitro , anemia , biochemistry
Anti‐Kell antibodies have been shown to suppress fetal erythropoiesis, but little is known about their effect on myelopoiesis. We analysed the effect of Kell‐related antibodies on granulocyte–macrophage colony‐forming units (CFU‐GM) growth in semisolid medium using peripheral blood mononuclear cells (PBMNCs) from haematologically normal individuals. In addition to its inhibitory effect on erythroid burst‐forming units (BFU‐E) growth, anti‐Kell antibodies significantly reduced CFU‐GM colony formation from Kell‐positive individuals but not from Kell‐negative donors. Moreover, anti‐cellano and anti‐Kp b antibodies also inhibited the growth of CFU‐GM from antigen‐positive MNCs. These data indicate that Kell is not restricted to erythroid blood cells, but is also expressed on myeloid progenitor cells.