Premium
Role of A and B blood group antigens in the expression of adhesive activity of von Willebrand factor
Author(s) -
Sarode Ravindra,
Goldstein Jack,
Sussman Ira I.,
Nagel Ronald L.,
Tsai HanMou
Publication year - 2000
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2000.02113.x
Subject(s) - antigen , von willebrand factor , abo blood group system , chemistry , microbiology and biotechnology , trypsin , platelet , enzyme , biochemistry , immunology , biology
ABO (H) blood group antigens are covalently linked to the oligosaccharide side‐chains of von Willebrand factor (VWF). In this study, we investigated the role of the A and B antigens in the expression of VWF adhesive activity. VWF of type A, B or O was purified from fresh frozen plasma. Presence of A or B antigen on the VWF was confirmed by enzyme‐linked immunosorbent assay (ELISA) and by immunoblotting with monoclonal anti‐A or anti‐B. The A or B antigen was also detected in the 48/52‐kDa fragment of the respective VWF after trypsin digestion. Removal of A antigen with α‐ N ‐acetylgalactosaminidase or B antigen with α‐galactosidase did not affect its multimer size or antigenic level, but decreased the ristocetin cofactor (RCoF) activity of the respective VWF by 33–39% ( P < 0·01–0·002). Removal of A or B antigen from VWF did not affect the binding of the VWF to immobilized type III collagen. A and B antigens were not detected in platelet VWF. These results indicate that AB structures play a role in platelet aggregating activity of VWF.