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Genetic instability in myelodysplastic syndrome: detection of microsatellite instability and loss of heterozygosity in bone marrow samples with karyotype alterations
Author(s) -
Maeck Lienhard,
Haase Detlef,
Schoch Claudia,
Hiddemann Wolfgang,
Alves Frauke
Publication year - 2000
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2000.02088.x
Subject(s) - loss of heterozygosity , microsatellite instability , biology , microsatellite , cytogenetics , chromosome instability , genetics , karyotype , chromosome , polymerase chain reaction , myelodysplastic syndromes , cancer research , bone marrow , immunology , gene , allele
Using a polymerase chain reaction (PCR)‐based approach, we examined the prevalence of loss of heterozygosity (LOH) and microsatellite instability (MSI) in relation to chromosomal imbalances in myelodysplastic syndrome (MDS). Two of 26 patients displayed MSI (8%), one of them at five loci. LOH was detected in six out of 26 cases (23%), predominantly involving markers IRF1 [5q31] and WT1 [11p]. Two patients displayed a corresponding chromosomal deletion by conventional cytogenetics. Supporting the mutator phenotype hypothesis, a significant coincidence of LOH, MSI and chromosome abnormalities was observed ( P < 0·025). Moreover, our data suggest that LOH represents an initial rather than a secondary genetic event in MDS, promoting genetic instability in a subset of patients.