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Ex vivo expansion of mature human neutrophils with normal functions from purified peripheral blood CD34 + haematopoietic progenitor cells
Author(s) -
Hino Masayuki,
Suzuki Kenichi,
Yamane Takahisa,
Sakai Nobuaki,
Kubota Hiroshi,
Koh Ki Ryang,
Ohta Kensuke,
Hato Fumihiko,
Kitagawa Seiichi,
Tatsumi Noriyuki
Publication year - 2000
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2000.02054.x
Subject(s) - haematopoiesis , biology , progenitor cell , granulocyte colony stimulating factor , ex vivo , immunology , granulocyte , cd34 , interleukin 3 , cytotoxic t cell , stem cell , microbiology and biotechnology , t cell , in vitro , immune system , antigen presenting cell , biochemistry , chemotherapy , genetics
Purified CD34 + haematopoietic progenitor cells were cultivated with stem cell factor, interleukin 3 (IL‐3), granulocyte–macrophage colony‐stimulating factor (GM‐CSF) and granulocyte CSF (G‐CSF) for 7 d, and thereafter non‐adherent cells were divided into two groups. Cells in one group (group A) were further cultivated for 7 d with four cytokines, and cells in the other group (group B) were further cultivated for 7 d with G‐CSF alone. On day 14, 220‐fold and 130‐fold increases in the numbers of non‐adherent cells were achieved for groups A and B respectively. These cell preparations contained 65% granulocytes for group A and 95% granulocytes for group B. These cells gained the ability to respond effectively with chemotaxis, phagocytosis and superoxide (O 2 − ) release. Cells in group B were appropriately primed by G‐CSF, GM‐CSF, tumour necrosis factor α and IL‐1β for enhanced release of . The responsiveness of these cells was identical to that of peripheral blood neutrophils, indicating that cells in group B may be in the resting state. In contrast, cells in group A were not primed by these cytokines for enhanced release of O 2 − and released a large amount of O 2 − spontaneously, indicating that cells in group A may be in the activated state. These findings indicate that mature neutrophils with normal functions were expanded ex vivo in group B and suggest that these cells could be used for possible autologous neutrophil transfusion to prevent bacterial infections during severe neutropenia after cytotoxic chemotherapy.