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Control of pH in human long‐term bone marrow cultures with low‐glucose medium containing zwitterion buffer lengthens the period of haemopoietic activity
Author(s) -
Tennant G. B.,
Truran L. N.,
BaileyWood R.,
Burnett A. K.
Publication year - 2000
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2000.02002.x
Subject(s) - andrology , bone marrow , cord blood , peripheral blood mononuclear cell , stromal cell , granulocyte , bicarbonate , haematopoiesis , colony forming unit , in vitro , biology , immunology , stem cell , medicine , endocrinology , biochemistry , microbiology and biotechnology , genetics , bacteria
Primary long‐term bone marrow cultures grown in 40 m m HEPES‐buffered McCoy's 5A medium produced granulocyte–macrophage colony‐forming units (CFU‐GM) for a median of 9 weeks compared with 7 weeks with CO 2 /bicarbonate‐buffered cultures. Reducing the medium glucose concentration (from 12·5 to 2·75 m m ) extended the culture longevity to 17 weeks. The median period of erythroid colony detection increased from 6 to 8 weeks. Secondary cultures (5 × 10 6 cord blood mononuclear cells seeded on irradiated stroma) showed statistically similar myeloid and erythroid longevity to primary cultures. Improved control of medium pH significantly improved the capacity of long‐term stromal layers to maintain stem cells in vitro .