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Human bone marrow stroma‐dependent cell line MOLP‐5 derived from a patient in leukaemic phase of multiple myeloma
Author(s) -
Matsuo Yoshinobu,
Drexler Hans G.,
Nishizaki Chiharu,
Harashima Akira,
Fukuda Shunichi,
Kozuka Teruhiko,
Sezaki Tatsuo,
Orita Kunzo
Publication year - 2000
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2000.01985.x
Subject(s) - bone marrow , stromal cell , microbiology and biotechnology , biology , plasma cell , cell culture , cd44 , antibody , immunoglobulin light chain , cancer research , pathology , immunology , cell , medicine , biochemistry , genetics
The novel multiple myeloma (MM) cell line MOLP‐5 and its homologous sister cell line B407, a lymphoblastoid cell line (LCL), were established from the peripheral blood of a 71‐year‐old Japanese patient with Bence‐Jones κ‐type multiple myeloma (stage IIIB with hyperammonaemia and hypercalcaemia). The growth of MOLP‐5 cells is constitutively dependent on bone marrow stroma (BST) cells; none of the cytokines tested nor the culture supernatant of the bone marrow stroma cells could support the growth of MOLP‐5. Wright–Giemsa‐stained MOLP‐5 cells showed typical plasma cell morphology with abundant cytoplasm and one to three nuclei. The immunoprofile of MOLP‐5 corresponds to that seen typically in primary MM cells: positive for cytoplasmic immunoglobulin (Ig) κ light chain, CD28, CD29, CD38, CD40, CD44, CD49d, CD54, CD56, CD58, CD71, CD138 and PCA‐1; the cells were negative for surface Ig and various other B‐cell, T‐cell and myelomonocyte‐associated immunomarkers. Interleukin 6 (IL‐6) receptor mRNA was found in the reverse transcriptase polymerase chain reaction (RT‐PCR) analysis. IL‐6 and IL‐10 could induce cellular proliferation in short‐term induction experiments. IL‐6 or IL‐10 production was not detected by specific enzyme‐linked immunoabsorbent assay (ELISA). MOLP‐5 cells expressed parathyroid hormone‐related protein (PTHrP) at the mRNA level. Cytogenetic analysis showed the typical t(11; 14) chromosome abnormality. The novel MOLP‐5 cell line together with the B407 B‐LCL sister line will be useful model systems in the investigation of the biology of MM.