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Enrichment of lineage − CD34 − cells using a newly developed filter system
Author(s) -
Nakamura Y.,
Ando K.,
Muguruma Y.,
Sumita M.,
Terashima S.,
Yoshida M.,
Yamashita Y.,
Hotta T.,
Kato S.
Publication year - 2000
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2000.01921.x
Subject(s) - cd34 , population , cell sorting , microbiology and biotechnology , cell culture , chemistry , cell , biology , stem cell , flow cytometry , biochemistry , medicine , genetics , environmental health
Recent studies have demonstrated the presence of human lineage − (lin − )CD34 − cells that are capable of differentiating into CD34 + cells in xenogeneic transplantation systems. We developed a new filter system that can enrich lin − CD34 − cells, a precursor cell population of CD34 + cells. The filter consists of polyethylene terephthalate non‐woven fabrics coated with hydrophilic polymer. The frequency of lin − CD34 − cells in the cell population after filtration was 7.45 ± 4.41%, a 16.8 ± 8.81‐fold enrichment compared with 0.49 ± 0.31% in the cell population before filtration. The mean recovery of lin − CD34 − cells was 48.57 ± 13.59% ( n = 15). Purified lin − CD34 − cells, obtained by sorting the filtrated cell population, acquired CD34 expression and colony‐forming activity after 7 d of culture. Our results indicate that this filter system is useful for isolating lin − CD34 − cells, including precursors of CD34 + cells, and will help further the study of lin − CD34 − cells.