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Proliferative involvement of ENX‐1 , a putative human Polycomb group gene, in haematopoietic cells
Author(s) -
Fukuyama Tomofusa,
Otsuka Teruhisa,
Shigematsu Hirokazu,
Uchida Naoyuki,
Arima Fumitou,
Ohno Yuju,
Iwasaki Hiromi,
Fukuda Takahiro,
Niho Yoshiyuki
Publication year - 2000
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2000.01914.x
Subject(s) - haematopoiesis , biology , gene , retinoic acid , stem cell , genetics , microbiology and biotechnology
Homeobox genes have important roles in haematopoiesis and are regulated in an activated state by the trithorax group ( trxG ) of genes. In a repressed state, they are regulated by the Polycomb group ( PcG ) of genes. ENX‐1, a putative human PcG gene product, interacts with the proto‐oncogene product Vav. We report an investigation of the role of ENX‐1 in human haematopoiesis. CD34 + cells mobilized to peripheral blood strongly expressed ENX‐1 . When stimulated to proliferate, both T and B lymphocytes rapidly up‐regulated ENX‐1 . ENX‐1 was expressed in all cell lines of the various lineages examined. When HL‐60 cells were differentiated to mature granulocytes with all‐ trans retinoic acid, ENX‐1 was down‐regulated. Moreover, ENX‐1 antisense oligodeoxynucleotide suppressed DNA synthesis in HL‐60 cells. Our data indicate that ENX‐1 is involved in the proliferation of both normal and malignant haematopoietic cells.