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The effect of granulocyte colony‐stimulating factor administration in healthy donors before bone marrow harvesting
Author(s) -
Machida Utako,
Tojo Arinobu,
Takahashi Satoshi,
Iseki Toru,
Ooi Jun,
Nagayama Hitomi,
Shirafuji Naoki,
Mori ShinIchiro,
Wada Yuka,
Ogami Kazuo,
Yamada Yoshitsugu,
Sakamaki Hisashi,
Maekawa Taira,
Tani Kenzaburo,
Asano Shigetaka
Publication year - 2000
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2000.01910.x
Subject(s) - granulocyte colony stimulating factor , bone marrow , medicine , granulocyte , cd34 , immunology , granulocyte macrophage colony stimulating factor , priming (agriculture) , andrology , stem cell , cytokine , chemotherapy , biology , botany , germination , genetics
To investigate whether granulocyte colony‐stimulating factor (G‐CSF) administration to donors before harvest may lighten the burden imposed on them and accelerate the bone marrow (BM) recovery, we administered 2 μg/kg/d of G‐CSF for five consecutive days before the marrow harvest. All of the donors tolerated the G‐CSF administration well without severe adverse events. After 5 d of G‐CSF treatment, CD34 + cells and granulocyte–macrophage colony‐forming units (GM‐CFU) in the donors' BM exceeded baseline values by 4.2‐fold (range 0.71–316) and 1.6‐fold (0.28–118) respectively. The concentration of total nucleated cells (× 10 7 /ml) in the graft increased from 1.61 (0.95–3.23) to 2.44 (1.27–4.01). Although we collected 1020 ml of BM and obtained 1.50 × 10 10 nucleated cells from unprimed donors, 940 ml of BM were sufficient to obtain 2.14 × 10 10 nucleated cells from primed donors. However, G‐CSF‐primed BM did not shorten the time to tri‐lineage engraftment and the duration of hospitalization compared with unprimed BM, although primed BM contained more CD34 + cells than baseline values. We consider that the advantages of BM priming are not the acceleration of BM recovery but rather the reduction of blood loss during BM harvesting.