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Spontaneous monoclonal immunoglobulin‐secreting peripheral blood mononuclear cells as a marker of disease severity in multiple myeloma
Author(s) -
Cordoba Franck,
LavabreBertrand Thierry,
Salhi Sharon Lynn,
Huguet Marie France,
Gerfaux Jacqueline,
Rossi Jean Francois,
Vendrell Jean Pierre
Publication year - 2000
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.2000.01899.x
Subject(s) - isotype , peripheral blood mononuclear cell , immunoglobulin light chain , antibody , monoclonal , microbiology and biotechnology , elispot , monoclonal antibody , multiple myeloma , polyclonal antibodies , monoclonal gammopathy of undetermined significance , bone marrow , immunology , b cell , antigen , medicine , biology , in vitro , cd8 , biochemistry
Peripheral blood from patients with multiple myeloma (MM) contains a small number of plasma cells related to the bone marrow tumour cells by their cytoplasmic immunoglobulin (Ig), their cell membrane antigen expression and/or their gene rearrangements, but hitherto the monoclonal Ig (M‐Ig) production by circulating cells has not been reported. Using a two‐colour ELISPOT assay, Ig‐secreting cells (Ig‐SCs) were detected in the blood of 28 MM and five Waldenstrom's macroglobulinaemia (WM) patients. The number of cells that spontaneously produced an Ig isotype similar to that of the M‐Ig in serum was greater than that of the other Ig‐SCs. MM patients presented an excess of circulating heavy‐chain (α or γ) Ig‐SCs (0.38% of the PBMC) with κ or λ light chains (0.48%) compared with the number of cells secreting the other heavy‐ (0.02%) and light‐chain isotypes (0.03%). WM patients also presented high numbers of cells secreting the μ‐heavy‐chain isotype (0.66%). The Ig synthesized in vitro was characterized as monoclonal, and the M‐Ig secretory capacity of the peripheral blood cells was similar to that observed for Ig‐SCs from polyclonal activated B cells in vivo . The number of these monoclonal cells was significantly increased in patients in an advanced stage of MM (I/II vs. III, P < 0.001) and correlated with the serum beta‐2 microglobulin concentration ( r = 0.69; P < 0.0003). The number of M‐Ig‐SCs in MM patients could be a useful marker for evaluating the progression of multiple myeloma.