Premium
Phenotypic and functional characteristics of monocyte‐derived dendritic cells from patients with myelodysplastic syndromes
Author(s) -
Matteo Rigolin Gian,
Howard Julie,
Buggins Andrea,
Sneddon Claire,
Castoldi Gianluigi,
Hirst William J. R.,
Mufti Ghulam J.
Publication year - 1999
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1999.01781.x
Subject(s) - myelodysplastic syndromes , clone (java method) , immunology , phenotype , biology , antigen , fluorescence in situ hybridization , bone marrow , genetics , chromosome , gene , dna
We have compared the phenotypic and functional characteristics of dendritic cells (DC) generated in vitro from the peripheral blood mononuclear fraction of myelodysplastic syndrome (MDS) patients (four refractory anaemia, four refractory anaemia with excess of blasts) with DCs generated in a similar way from eight healthy donors. After 10 d of culture in the presence of GM‐CSF and IL‐4, reduced numbers and percentages of DCs were obtained in MDS subjects. MDS DCs exhibited significantly lower expression of CD1a, CD54, CD80 and MHC class II molecules. Their ability to stimulate T lymphocytes in an allogeneic mixed leucocyte reaction was reduced in comparison to normal subjects. Furthermore, MDS DCs also showed a reduced receptor‐mediated endocytosis as demonstrated by FITC‐dextran uptake. Simultaneous fluorescence in situ hybridization (FISH) and immunophenotypic analysis demonstrated that MDS DCs have the same cytogenetic abnormality of the malignant clone. Taken together these findings indicate that, in MDS, DCs are part of the malignant clone and exhibit a deficient antigen uptake and presentation.