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Phosphatidylserine‐related adhesion of human erythrocytes to vascular endothelium
Author(s) -
Closse Christèle,
DacharyPrigent Jeanne,
Boisseau Michel R.
Publication year - 1999
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1999.01718.x
Subject(s) - phosphatidylserine , umbilical vein , endothelium , adhesion , annexin , cell adhesion , red blood cell , chemistry , platelet , microbiology and biotechnology , phospholipid , immunology , biology , cell , biochemistry , in vitro , endocrinology , organic chemistry , membrane
In pathological conditions such as sickle cell disease, falciparum malaria and diabetes, an abnormal adherence of erythrocytes to endothelium is concomitant with loss of phospholipid asymmetry resulting in phosphatidylserine (PS) exposure. We have investigated the involvement of PS in this interaction by studying adhesion of human erythrocytes, treated with Ca 2+ ‐ionophore A23187 in combination with N‐ethylmaleimide, to human umbilical vein endothelial cells in a flow‐based assay. Results showed that erythrocytes which exposed PS, massively adhered to HUVEC in a Ca 2+ ‐dependent manner. This adhesion was inhibited by PS liposomes and by annexin V, giving clear evidence of the PS dependence of these interactions.