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Kaposi's sarcoma‐associated herpesvirus (KSHV/HHV‐8) DNA sequences are absent in leukapheresis products and ex vivo expanded CD34 + cells from multiple myeloma patients
Author(s) -
De Greef Catherine,
Van De Voorde Wendy,
Bakkus Marleen,
Corthals Jurgen,
Heirman Carlo,
Schots Rik,
Lacor Patrick,
Van Camp Ben,
Van Riet Ivan
Publication year - 1999
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1999.01628.x
Subject(s) - leukapheresis , ex vivo , cd34 , progenitor cell , virology , bone marrow , multiple myeloma , sarcoma , kaposi's sarcoma associated herpesvirus , myeloid , cancer research , biology , immunology , in vivo , stem cell , medicine , virus , pathology , herpesviridae , viral disease , microbiology and biotechnology
Recently it was reported that Kaposi's sarcoma‐associated herpesvirus (KSHV/HHV‐8) infects bone marrow (BM) dendritic cells (DC) in multiple myeloma (MM) patients and therefore might play a role in MM development. Because of the use of myeloid growth factors like GM‐CSF and G‐CSF for the mobilization of peripheral blood progenitor cells (PBPC), the subsequent increase of DC precursors might imply a risk for KSHV contamination in PBPC grafts. Therefore, in this study leukapheresis products and ex vivo cultured CD34 + cell suspensions were analysed. KSHV DNA could not be amplified in any of them.