Premium
The cyclin‐dependent kinase inhibitors p18 INK4c and p19 INK4d are highly expressed in CD34 + progenitor and acute myeloid leukaemic cells but not in normal differentiated myeloid cells
Author(s) -
Tschan M. P.,
Peters U. R.,
Cajot J. F.,
Betticher D. C.,
Fey M. F.,
Tobler A.
Publication year - 1999
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1999.01617.x
Subject(s) - cd34 , progenitor cell , myeloid , haematopoiesis , cancer research , biology , progenitor , cellular differentiation , stem cell , microbiology and biotechnology , genetics , gene
Cyclin‐dependent kinase inhibitors (CKIs) are important for the differentiation of cells in various tissues. In acute myeloid leukaemia (AML) the cells accumulate at particular stages of myeloid maturation. We therefore analysed the expression pattern of different CKIs in fresh samples of AML patients and compared it with that in CD34 + progenitor and normal differentiated myeloid cells. Competitive RT‐PCR and Western analysis revealed a significantly higher expression of p18 INK4c and p19 INK4d in leukaemic and CD34 + progenitor cells than in granulocytes and monocytes. A different pattern was seen for p27 Kip1 and p57 Kip2 expression being low in leukaemic cells but high in normal immature and differentiated cells. No marked differences were found in p15 INK4b and p21 Cip1 mRNA expression between leukaemic and CD34 + progenitor or mature myeloid cells. Our findings therefore indicate that high expression of p18 INK4c and p19 INK4d in haemopoietic progenitor and leukaemic blast cells may contribute to the premature differentiation block seen in AML.