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A combination of megakaryocyte growth and development factor and interleukin‐1 is sufficient to culture large numbers of megakaryocytic progenitors and megakaryocytes for transfusion purposes
Author(s) -
Van Den Oudenrijn Sonja,
De Haas Masja,
Calafat Jero,
Van Der Schoot C. Ellen,
Von Dem Borne Albert E. G. KR.
Publication year - 1999
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1999.01569.x
Subject(s) - megakaryocyte , progenitor cell , interleukin 3 , immunology , progenitor , growth factor , biology , microbiology and biotechnology , medicine , stem cell , receptor , immune system , t cell , antigen presenting cell
Chemotherapy‐induced thrombocytopenia is a major risk factor in cancer treatment. The transfusion of autologous ex vivo expanded megakaryocytes could be a new therapy to shorten the period of thrombocytopenia. Therefore we investigated, in a liquid culture system, the effect of various cytokine combinations composed of pegylated megakaryocyte growth and development factor (PEG‐rHuMGDF), interleukin‐1 (IL‐1), IL‐3, IL‐6, IL‐11 and stem cell factor (SCF) on the proliferation and differentiation of CD34 + cells, in order to define the most optimal and minimum levels of cytokine combinations for megakaryocyte expansion. Besides PEG‐rHuMGDF, IL‐1 was found to be important for optimal megakaryocyte expansion. Depletion of either SCF, IL‐6 or IL‐11 did not exert a large effect, but the absence of IL‐1 strongly diminished the number of megakaryocytic cells. Addition of IL‐3 to the combination PEG‐rHuMGDF, IL‐1, IL‐6, IL‐11 and SCF significantly reduced the number of megakaryocyte progenitors (CD34 + CD41 + cells) and the number of CFU‐Meg. Furthermore, we found a strong correlation between the number of CD34 + CD41 + cells and the number of CFU‐Meg obtained after 8 d culture. Our study shows that optimal ex vivo expansion of megakaryocytes is achieved by the combination of PEG‐rHuMGDF and IL‐1. The numbers of megakaryocytes and megakaryocyte progenitors (CD34 + CD41 + ) obtained in our liquid culture system with the growth factor combination PEG‐rHuMGDF and IL‐1 are suitable for transfusion purposes.