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Early diagnosis of central nervous system aspergillosis using polymerase chain reaction, latex agglutination test, and enzyme‐linked immunosorbent assay
Author(s) -
Kami Masahiro,
Ogawa Seishi,
Kanda Yoshinobu,
Tanaka Yuji,
Machida Utako,
Matsumura Tomoko,
Sakamaki Hisashi,
Hirai Hisamaru
Publication year - 1999
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1999.01542.x
Subject(s) - latex fixation test , polymerase chain reaction , aspergillosis , central nervous system , medicine , direct agglutination test , agglutination (biology) , virology , immunology , biology , antibody , serology , biochemistry , gene
To investigate the usefulness of examination of cerebrospinal fluids (CSF) in the diagnosis of central nervous system (CNS) aspergillosis, we examined five patients with either brain abscesses or cerebral infarctions and 11 control patients. CSF samples were subjected to enzyme‐linked immunosorbent assay (EIA), latex agglutination test (LA) and polymerase chain reaction (PCR). Cultures of CSF samples were negative in all the patients, but PCR, EIA and LA were positive in five, four and four patients with CNS aspergillosis, respectively. None of these tests were positive in the control patients. CSF examination may be beneficial in the diagnosis of CNS aspergillosis.