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Repeated peripheral stem cell mobilization in healthy donors: time‐dependent changes in mobilization efficiency
Author(s) -
Tichelli André,
Passweg Jakob,
Hoffmann Till,
Gregor Michael,
Kühne Thomas,
Favre Geneviève,
WodnarFilipowicz Alexandra,
Gratwohl Alois
Publication year - 1999
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1999.01518.x
Subject(s) - mobilization , peripheral , medicine , stem cell , peripheral blood , biology , political science , microbiology and biotechnology , law
Mobilization of peripheral blood stem cells was analysed in 10 consecutive healthy donors undergoing repeated stem cell mobilization for allogeneic transplantation. Donors received recombinant G‐CSF at a dose of 10 μg/kg/d for both mobilizations. Collection of stem cells was started on day 5 of G‐CSF administration. To compare the efficiency of first and second mobilization, we determined the leucocyte and CD34 + cell counts in peripheral blood, and the yield of nucleated cells and CD34 + cell counts in the apheresis product. CD34 + cell numbers in peripheral blood were (median) 81.2 × 10 6 /l during the first and 50.4 × 10 6 /l during the second mobilization ( P = 0.007). Likewise, CD34 + cells in the apheresis product decreased from 319.8 × 10 6 to 275.7 × 10 6 ( P = 0.02). Decrease in CD34 + cell counts in peripheral blood and in the apheresis product was associated with the time interval between first and second mobilization. In a regression analysis there was a correlation between the ratios of CD34 + cell counts of first and second mobilization and the inverse of time interval between procedures ( r 2 = 0.51 peripheral blood; r 2 = 0.74 apheresis product). Thus, stem cell yield is reduced when healthy donors receive repeated mobilization within a short time. Nevertheless, an adequate number of stem cells may repeatedly be mobilized within 2 months.