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SDF‐1 suppresses cytokine‐induced adhesion of human haemopoietic progenitor cells to immobilized fibronectin
Author(s) -
Gotoh Akihiko,
Reid Suzanna,
Miyazawa Keisuke,
Broxmeyer Hal E.
Publication year - 1999
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1999.01517.x
Subject(s) - progenitor cell , fibronectin , microbiology and biotechnology , homing (biology) , cytokine , chemokine , biology , haematopoiesis , extracellular matrix , cell adhesion , immunology , cd34 , stem cell , chemistry , inflammation , cell , biochemistry , ecology
The process of haemopoietic cell homing to the microenviroment includes migration and adhesion. SDF‐1 is a C‐X‐C chemokine that acts as a chemoattractant for haemopoietic progenitors. Adhesion of haemopoietic progenitors to immobilized fibronectin is up‐regulated by stimulation with cytokines. We assessed the effects of SDF‐1 on cytokine‐induced adhesion of progenitor cells to fibronectin. In factor‐dependent human MO7e cells and primary CD34 + cord blood cells, treatment with SDF‐1 dose‐dependently suppressed cytokine‐induced adhesion. Pretreatment with pertussis toxin reversed adhesion‐inhibition, suggesting that activation of G‐coupled proteins are involved in the intracellular signalling of this process. These data suggest that SDF‐1 not only acts as a chemoattractant but also participates in modulating adhesiveness of haemopoietic progenitors to extracellular matrix components.