Premium
Analysis of progenitor cell involvement in B‐CLL by simultaneous immunophenotypic and genotypic analysis at the single cell level
Author(s) -
Gahn Benedikt,
Wendenburg Britta,
Troff Carmen,
Neef Juliane,
Grove Doris,
Haferlach Torsten,
Hiddemann Wolfgang,
Wörmann Bernhard
Publication year - 1999
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1999.01471.x
Subject(s) - progenitor cell , cd34 , bone marrow , biology , progenitor , stem cell , trisomy , immunology , cd38 , b cell , cancer research , pathology , medicine , antibody , genetics
B‐cell chronic lymphocytic leukaemia (B‐CLL) results from the clonal expansion of mature B lymphocytes. The detection of leukaemia‐associated genetic markers in CD34‐positive progenitor cells in a subset of B‐CLL patients suggests that malignant transformation in B‐CLL occurs in an immature progenitor cell compartment. To further quantify the percentage of B‐CLL patients with genetically aberrant progenitor cells we have investigated CD34 + bone marrow cells in 11 B‐CLL patients at the single cell level by simultaneous genetic and immunophenotypic analysis (FICTION). In five patients with trisomy 12, CD34 + haemopoietic progenitor cells were detectable on bone marrow smears. In one patient with trisomy 12, CD34 + progenitor cells were isolated by FACS sorting. In all six patients trisomy 12 was not found in the CD34 + cells. Progenitor cells were also analysed in three patients with Rb‐deletion and in two patients with deletion of p53. In all patients the genetic marker was not detected in the CD34 + cells. In conclusion, we did not find genetically aberrant progenitor cells in this group of B‐CLL patients. These results suggest that the subset of B‐CLL patients with genetically aberrant CD34 + cells may be very small. This is of significance for our understanding of B‐CLL biology and for future strategies using autologous stem cell transplantation.