Premium
Myeloid differentiation of human cord blood CD34 + cells during ex vivo expansion using thrombopoietin, flt3‐ligand and/or granulocyte‐colony stimulating factor
Author(s) -
Yoo EunSeon,
Ryu KyungHa,
Park HaeYoung,
Seong ChuMyung,
Chung WhaSoon,
Kim SeungCheol,
Choi YongMook,
Hahn MyongJoon,
Woo SoYoun,
Seoh JuYoung
Publication year - 1999
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1999.01456.x
Subject(s) - thrombopoietin , cord blood , ex vivo , cd64 , cd34 , cd16 , biology , microbiology and biotechnology , myeloid , flow cytometry , integrin alpha m , granulocyte , immunology , stem cell factor , haematopoiesis , chemistry , in vivo , stem cell , antigen , cd3 , cd8
We investigated the phenotypic changes of human umbilical cord blood (CB) CD34 + cells during ex vivo expansion using thrombopoietin (TPO), flt3‐ligand (FL), and/or granulocyte‐colony stimulating factor (G‐CSF). During ex vivo expansion of CD34 + cells isolated from human CB for up to 5 weeks, surface expression of molecules on the cultured cells including CD64 (FcγRI), CD32 (FcγRII), CD16 (FcγRIII), CD11b (MAC‐1) and CD18 (β 2 ‐integrin) was analysed by flow cytometry along with simultaneous measurement of apoptosis by 7‐aminoactinomycin D staining method. CD64, CD32 and/or CD18 expressing cells appeared in the cultures both with and without the addition of G‐CSF until the tenth day. However, without G‐CSF, CD16 + fractions did not appear and CD11b + fractions were not maintained. With G‐CSF, the CD16 + or CD11b + fractions appeared only from the second week. These results suggest that G‐CSF is necessary for the late stage of myeloid maturation during which CD16 and CD11b are expressed.