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Transport of 14 C‐deferiprone in normal, thalassaemic and sickle red blood cells
Author(s) -
Shalev Oded,
Hileti Dona,
Nortey Philip,
Hebbel Robert P.,
Hoffbrand Victor A.
Publication year - 1999
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1999.01447.x
Subject(s) - deferiprone , incubation , chemistry , intracellular , extracellular , cytosol , red blood cell , efflux , hemoglobinopathy , membrane , incubation period , biochemistry , biophysics , deferoxamine , medicine , hemolytic anemia , biology , enzyme
The transport of deferiprone (L1) in normal (N), sickle (S) and thalassaemic (T) red blood cells (RBC) was determined by incubation with 14 C‐L1 at 37°C. Following incubation with 0.5 m m 14 C‐L1 for 4 h, the intracellular concentration of L1 in T RBC was 3 times higher than was found extracellularly. In contrast, no concentration gradient across N and S RBC membranes was detected. Efflux studies showed that T RBC released only 17 ± 2% of 14 C‐L1 into the extracellular space. We hypothesize that L1 accumulation in T RBC results from their high content of chelatable iron and formation of large, hydrophilic L1–Fe(III) complexes trapped within the cytosol.