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Expression of CD41 and c‐ mpl does not indicate commitment to the megakaryocyte lineage during haemopoietic development
Author(s) -
Basch Ross S.,
Zhang XinMin,
Dolzhanskiy Alexandr,
Karpatkin Simon
Publication year - 1999
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1999.01446.x
Subject(s) - megakaryocyte , thrombopoietin , cd34 , haematopoiesis , progenitor cell , population , myeloid , biology , cd38 , bone marrow , microbiology and biotechnology , clonogenic assay , stem cell , immunology , chemistry , cell culture , genetics , medicine , environmental health
Haemopoietic progenitors with the phenotype expected of early megakaryocyte precursors (CD34 + CD41 + ) were isolated from normal human bone marrow or induced in culture from CD34 + CD41 − bone marrow cells by treatment with thrombopoietin (TPO) or IL‐3. We found that although this population included the majority of cells that can form CFU‐MK in culture, it also contained both erythroid and myeloid progenitors. The clonogenic potential of the CD34 + CD41 + ‐induced cells was greater than that of isolated CD34 + CD41 + cells in that the isolated cells only formed CFU‐MK and BFU‐e, whereas the induced cells formed myeloid colonies as well. Glycophorin was found on isolated CD34 + CD41 + cells, not on induced cells. Its presence distinguished between MK and erythroid progenitors. Separation of a CD34 + CD41 + glycophorin A + population resulted in the isolation of a highly purified population of BFU‐e. A major portion of the cells that expressed CD34 + CD41 + , in either cohort, were of the erythroid lineage. True MK progenitors were present in the CD34 + population in greater proportion than in whole marrow and were further enriched amongst CD34 + populations that expressed CD41. The presence of the thrombopoietin (TPO) receptor, c‐ mpl , did not correlate with inducibility of the gpIIbIIIa complex since essentially all CD34 + progenitors, including the earliest identifiable human haemopoietic progenitors (CD34 + CD38 − cells), expressed c‐mpl mRNA detectable by PCR regardless of their ultimate fate. Thus neither the expression of CD41 nor the expression of c‐mpl was predictive of commitment to the MK lineage.