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An improved micro‐method for obtaining chromosome preparations from individual haemopoietic colonies
Author(s) -
Kadhim M. A.,
Pocock D. A.,
Lorimore S. A.,
Wright E. G.
Publication year - 1999
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1999.01428.x
Subject(s) - staining , chromosome , chromosome analysis , biology , karyotype , chromosome number , microbiology and biotechnology , andrology , genetics , medicine , gene
A robust method for obtaining chromosome preparations from individual haemopoietic colonies in semi‐solid media is described. The accumulation of metaphases and the hypotonic treatment of cells were carried out in the culture dish and individual colonies were transferred onto poly‐ l ‐lysine‐treated slides and fixed stepwise prior to staining. The technique produced high yields of well‐spread metaphases facilitating clonal cytogenetic analysis.