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Establishment of a near‐triploid human B‐cell lymphoma cell line with t(14;18) and a p53 gene point mutation
Author(s) -
Yamaguchi Hiroki,
Inokuchi Koiti,
Hanawa Hideki,
Tanosaki Sakae,
Shinozawa Isao,
Matuoka Hiroki,
Dan Kazuo
Publication year - 1999
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1999.01402.x
Subject(s) - chromosomal translocation , biology , point mutation , lymphoma , mutation , gene , cancer research , population , cell culture , microbiology and biotechnology , cell , genetics , immunology , medicine , environmental health
We report a rare large B‐cell non‐Hodgkin's lymphoma having a characteristic near‐triploid cell population with add(17)(p22) and t(14;18)(q32;q21) translocation. We also established and characterized a new cell line (TK cell) derived from the present lymphoma. A codon 180 mutation (GAG → GAT) in the p53 gene was detected. t(14;18)(q32;q21) was revealed juxtaposition of the bcl‐2 and J H genes. Immunoprecipitation analyses of p53 and bcl‐2 revealed that abnormality of the p53 protein and aberrant bcl‐2 expression, which may protect cells from apoptosis, may be critical to the development of leukaemogenesis exhibiting near‐triploid chromosomes.