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Survival of human leukaemic B‐cell precursors is supported by stromal cells and cytokines: association with the expression of bcl‐2 protein
Author(s) -
Nishii Kazuhiro,
Katayama Naoyuki,
Miwa Hiroshi,
Shikami Masato,
Masuya Masahiro,
Shiku Hiroshi,
Kita Kenkichi
Publication year - 1999
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1999.01380.x
Subject(s) - stromal cell , cd19 , cd20 , b cell , cancer research , biology , antibody , microbiology and biotechnology , immunology , chemistry
We searched for cytokines with the potential to support the survival of human B‐cell precursor acute lymphoblastic leukaemia (pre‐B ALL) cells. 47 patients with pre‐B ALL were classified into four stages: stage I, CD19 + CD10 − CD20 − ; stage II, CD19 + CD10 + CD20 − ; stage III, CD19 + CD10 + CD20 + cytoplasmic μ‐heavy chain (cμ) − ; stage IV, CD19 + CD10 + CD20 + cμ + . Interleukin (IL)‐3 receptor α chain (IL‐3Rα) was expressed in all stages, whereas the expressions of IL‐7Rα and IL‐2Rα were pronounced in stages IV and II, respectively. Neither IL‐3, IL‐7 nor IL‐2 supported the survival of pre‐B ALL cells. When pre‐B ALL cells were layered on stromal, MS‐10, cells, viability of the pre‐B ALL cells increased. Addition of IL‐3 to culture containing MS‐10 cells enhanced the survival of pre‐B ALL cells in all cases, whereas addition of IL‐7 augmented the survival of pre‐B ALL cells of some cases of stage III and all cases of stage IV. The survival of pre‐B ALL cells was also supported by the conditioned media of MS‐10 cells. Stromal‐cell‐derived factor 1 (SDF‐1) supported the survival of pre‐B ALL cells. Effects of the conditioned media of MS‐10 cells were abrogated by an anti‐SDF‐1 neutralizing antibody. The extent of survival of pre‐B ALL cells supported by stromal cells and IL‐3 and IL‐7, correlated with the expression level of bcl‐2 protein. The effects of stromal cells may be in part related to SDF‐1.