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Molecular characterization of deletion at 11q22.1‐23.3 in mantle cell lymphoma
Author(s) -
Monni Outi,
Zhu Ying,
Franssila Kaarle,
Oin Riikka,
Höglund Pia,
Elonen Erkki,
Joensuu Heikki,
Knuutila Sakari
Publication year - 1999
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1999.01257.x
Subject(s) - contig , biology , yeast artificial chromosome , breakpoint , mantle cell lymphoma , fluorescence in situ hybridization , genetics , microbiology and biotechnology , gene mapping , lymphoma , deletion mapping , gene , chromosome , genome , immunology
Chromosomal deletions at 11q21‐23 have recently been reported to be common aberrations in mantle cell lymphoma (MCL). To characterize the structure of the deletion, we studied 41 cases of MCL by fluorescence in situ hybridization using a YAC contig, which spans the region at 11q22.1‐23.3. 17 MCLs were studied using a set of 20 yeast artificial chromosomes (YACs) in a contig, and nine of these cases showed deletion of 11q22‐23. The deletion spanned several megabases in all but one case, where only YAC 755b11 at 11q23.1, covering approximately a 1.6 Mb of DNA, was deleted. Analysis of additional 24 MCLs with YAC 755b11 revealed the deletion in 49% of all cases (20/41). The deleted region at 11q22.1‐23.3 was discontinuous in five lymphomas and in the majority of the cases the distal breakpoint occurred between YACs 785e12 and 911f2 at 11q23.3. We conclude that the deletion of 11q22‐23 and particularly the deletion of YAC 755b11 are very common in MCL and may be important in the genesis or progression of the disease.