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Antibody‐mediated inhibition of the human alloimmune response to platelet transfusion in Hu‐PBL‐SCID mice
Author(s) -
Crow Andrew R.,
Freedman John,
Hannach Barbara,
Blanchette Victor,
Lazarus Alan H.
Publication year - 1999
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1999.01237.x
Subject(s) - platelet , immunology , medicine , antibody , human leukocyte antigen , isoantibodies , platelet transfusion , immune system , antigen
Severe combined immune deficient (SCID) mice were engrafted with human (Hu) peripheral blood lymphocytes (PBL) from a previously alloimmunized donor and transfused with HLA‐mismatched platelets. We have previously shown this to be a useful model for platelet transfusion. These engrafted mice (Hu‐PBL‐SCID mice) produced high levels of alloantibody in response to standard platelet preparations. However, when the first platelet challenge was presensitized with anti‐HLA antibody and then transfused there was a marked reduction in the amount of alloantibody produced to five subsequent untreated platelet transfusions. Platelets pretreated with platelet‐specific anti‐HPA‐1a (PL A1 ) sera did not induce a decrease in the anti‐HLA alloantibody response. This demonstrated that platelet‐induced HLA alloimmunization can be blocked by anti‐HLA antibody‐sensitized cells.