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A reverse dot‐blot method for rapid detection of non‐deletion α thalassaemia
Author(s) -
CHAN VIVIAN,
YAM IRENE,
CHEN F. E.,
CHAN T. K.
Publication year - 1999
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1999.01221.x
Subject(s) - dot blot , oligonucleotide , microbiology and biotechnology , gene , southern blot , allele , biology , thalassemia , genetics
A reverse dot blot method based on membrane‐bound allele‐specific oligonucleotides as hybridization targets for amplified α‐gene fragments has been developed for the rapid detection of four non‐deletion α thalassaemia defects found in the Chinese. Since these non‐deletion defects account for 22.8% of haemoglobin H disease, a sensitive, specific and rapid screening method should be of value.