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Pre‐treatment of peripheral blood progenitor cells with macrophage inflammatory protein‐1α induces prolonged survival of early progenitor cells over 6 weeks of long‐term culture
Author(s) -
Egger, Dietmar,
Günther Christine,
Helbig Werner,
Schulze Elisabeth
Publication year - 1998
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1998.01105.x
Subject(s) - progenitor cell , progenitor , peripheral blood , macrophage , medicine , immunology , cancer research , stem cell , chemistry , biology , microbiology and biotechnology , in vitro , biochemistry
It has previously been shown that a combination of macrophage inflammatory protein‐1α (MIP‐1α) and interleukin (IL)‐3 maintained human bone marrow (BM)‐derived long‐term culture‐initiating cells (LTC‐IC) for at least 8 weeks in vitro . We investigated colony‐ and cobblestone area‐formation potential of peripheral blood progenitor cells (PBPC) at week 6 of long‐term culture (LTC) in the absence of exogenous MIP‐1α, but using cells which had been pre‐incubated in the presence of MIP‐1α for 40 h in liquid culture. The recovery of colony‐forming cells (CFC) and cobblestone‐area‐forming cells (CAFC) after pre‐incubation of PBPC with MIP‐1α was up to threefold higher ( P < 0.05, n =5) than that of untreated controls. These results demonstrate that short‐term pre‐treatment of PBPC with MIP‐1α induces long‐lasting survival effects on early PBPC‐derived progenitors in vitro .