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Activation of the human delta‐globin gene promoter in primary adult erythroid cells
Author(s) -
Tang Delia C.,
Rodgers Griffin P.
Publication year - 1998
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1998.01052.x
Subject(s) - microbiology and biotechnology , caat box , biology , erythroblast , gene , promoter , binding site , globin , gene expression , hypersensitive site , genetics , haematopoiesis , stem cell
Restoration of the CCAAT box or insertion of an erythroid Krüppel‐like factor (EKLF) binding site in the delta promoter activates its expression in several erythroid cell lines. We extended these studies using a novel primary human adult erythroid cell ( h AEC) system to investigate these effects at the late erythroblast stage. Restoration of the CCAAT box at −70 bp, or insertion of an EKLF binding site at −85 bp or −95 bp in the promoter significantly increased delta globin gene expression in h AEC. Our results demonstrate that the altered CCAAT box (CCAAC) and the lack of an EKLF binding site in δ‐globin contribute to its low level of expression in the h AEC model as well.