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Near‐haploid common acute lymphoblastic leukaemia of childhood with a second hyperdiploid line: a DNA ploidy and fluorescence in‐situ hybridization study
Author(s) -
Ma S. K.,
Chan G. C. F.,
Wan T. S. K.,
Lam C. K.,
Ha S. Y.,
Lau Y. L.,
Chan L. C.
Publication year - 1998
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1998.01044.x
Subject(s) - ploidy , fluorescence in situ hybridization , in situ hybridization , in situ , cancer research , biology , microbiology and biotechnology , medicine , genetics , chemistry , chromosome , gene , gene expression , organic chemistry
Near‐haploidy is a rare cytogenetic finding in childhood acute lymphoblastic leukaemia (ALL) and is associated with a poor prognosis. A second hyperdiploid line, occurring presumably by endoreduplication of the near‐haploid stemline, is often observed. We present a case of common ALL in relapse characterized morphologically by a dual population of small and large lymphoblasts. Cytogenetic analysis supplemented with fluorescence in‐situ hybridization (FISH) studies localized near‐haploidy and hyperdiploidy to the small and large blast population respectively. DNA ploidy determination confirmed two abnormal clones with near‐haploidy as the predominant one. A novel t(9;12)(q11;q13) was present in the near‐haploid clone and was duplicated in the hyperdiploid clone. This finding identified cells bearing near‐haploidy to be the clonogenic population following malignant transformation and confirmed endoreduplication as the mechanism for the presence of associated hyperdiploidy.