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Identification of new type 2B von Willebrand disease mutations: Arg543Gln, Arg545Pro and Arg578Leu
Author(s) -
Hilbert L.,
Gaucher C.,
Abgrall J. F.,
Parquet A.,
Trzeciak C.,
Mazurier C.
Publication year - 1998
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1046/j.1365-2141.1998.01040.x
Subject(s) - von willebrand disease , identification (biology) , medicine , disease , genetics , von willebrand factor , biology , computational biology , immunology , platelet , botany
We report the identification in five patients (three families) affected with type 2B von Willebrand disease (VWD) of three heterozygous nucleotide substitutions at the codon for arginine 543, 545 and 578 of the mature von Willebrand factor (VWF) subunit resulting in a glutamine, proline and leucine substitution, respectively. These mutations are located in the A1 loop where prevalent type 2B mutations (Arg543Trp, Arg545Cys and Arg578Gln) have been already identified at the same positions. By in vitro mutagenesis of full‐length cDNA of VWF and transient expression in Cos‐7 cells, we have shown that the six corresponding mutated recombinant VWFs (Gln543, Trp543, Cys545, Pro545, Leu578 and Gln578 rVWF) exhibited quantitatively normal expression and normal multimeric pattern but increased ristocetin‐ and botrocetin‐induced binding to platelets as compared with that for wild‐type rVWF. The two mutations at position 545 induced the greatest reactivity for GPIb of corresponding rVWFs as compared to the two mutations at positions 543 and 578.